CD8 is part of the Ig superfamily that expresses primarily as CD8α homodimers. CD8α is a 32-34 kD type I glycoprotein that can also form heterodimers with CD8β. Clone 2.43 recognizes an epitope on mouse CD8a.
| Catalog | IV0104 |
| Product Name | InVivoPro Anti-Mouse CD8α in vivo antibody, Clone 2.43 |
| Size | 1mg/5mg/25mg/50mg/100mg |
| Isotype | Rat IgG2b |
| Clone | 2.43 |
| Target | CD8α |
| Other Names | CD8 alpha chain，Ly 2.2，Ly-35 |
| Isotype Control Catalog Code | IV0106 or Rat IgG2b Isotype Control |
| Dilution Buffer | PBS, pH 7.2, contains no stabilizers or preservatives |
| Reactivity | Mouse |
| Host Species | Rat |
| How much antibody to use in vivo | 200-500 μg per mouse; or 10 mg/kg. This range is based off the most recent publication data using the 2.43 clone in vivo. Each investigator should determine their own optimal working dilution for specific applications. |
| Background(ichorbio) | CD8 is part of the Ig superfamily that expresses primarily as CD8α homodimers. CD8α is a 32-34 kD type I glycoprotein that can also form heterodimers with CD8β. Clone 2.43 recognizes an epitope on mouse CD8a. |
| Applications | In vivo CD8+ T cell depletion, Flow cytometry |
| Purification | protein A or G |
| Storage | This antibody is stable for at least 2 months when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at -20°C or -80°C. Avoid repeated freeze thaw cycles. |
| Shipping | 2-8°C with blue ice |
| Concentration | Lot specific, generally ≥ 5.0 mg/ml |
| Shelf Life | 12 months from the date of receipt if stored as recommended |
| Formulation | PBS Buffer, PH 7.2, with no carrier protein, or preservatives. |
| Sterility | 0.2 μM filtered |
| Endotoxin | ≤ 1.0 EU/mg, by the LAL method |
| Purity | 99% |
Sade-Feldman, Moshe et al. "Defining T Cell States Associated with Response to Checkpoint Immunotherapy in Melanoma",Cell 175.4 (2018): 998-1013.e20.
Krishnamurty, Akshay T. et al. "LRRC15 + Myofibroblasts Dictate the Stromal Setpoint to Suppress Tumour Immunity",Nature 611.7934 (2022): 148-154.
Fan, Hao et al. "Trans-vaccenic Acid Reprograms CD8+ T Cells and Anti-Tumour Immunity.",NATURE 623.7989 (2023): 1034-+.
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