CTLA-4 is a 33 kD member of the Ig superfamily similar to CD28 in amino acid sequence, structure, and genomic organization. CTLA-4 is a protein receptor that functions as an immune checkpoint and downregulates immune responses. It is involved in the development of protective immunity and thymocyte regulation, in addition to the induction and maintenance of immunological tolerance. CTLA-4 has therapeutic potential both as an agonist to reduce immune activity, and an antagonist to increase immune activity.
| Catalog | IV0109 |
| Product Name | InVivoPro Anti-Mouse CTLA-4 (CD152) in vivo antibody, Clone 9D9 |
| Size | 1mg/5mg/25mg/50mg/100mg |
| Isotype | Mouse IgG2b |
| Clone | 9D9 |
| Target | CTLA-4 |
| Other Names | CD152, Cytotoxic T Lymphocyte-Associated Antigen-4, Ly-56 |
| Isotype Control Catalog Code | Mouse IgG2b Isotype Control |
| Dilution Buffer | PBS, pH 7.2, contains no stabilizers or preservatives |
| Reactivity | Mouse |
| Host Species | Mouse |
| How much antibody to use in vivo | 200-500 μg per mouse; or 10-20 mg/kg. This range is based off the most recent publication data using the 9D9 clone in vivo. Each investigator should determine their own optimal working dilution for specific applications. |
| Background(ichorbio) | CTLA-4 is a 33 kD member of the Ig superfamily similar to CD28 in amino acid sequence, structure, and genomic organization. CTLA-4 is a protein receptor that functions as an immune checkpoint and downregulates immune responses. It is involved in the development of protective immunity and thymocyte regulation, in addition to the induction and maintenance of immunological tolerance. CTLA-4 has therapeutic potential both as an agonist to reduce immune activity, and an antagonist to increase immune activity. |
| Applications | In vivo CTLA-4 neutralization, Western blot |
| Purification | protein A or G |
| Storage | This antibody is stable for at least 2 months when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at -20°C or -80°C. Avoid repeated freeze thaw cycles. |
| Shipping | 2-8°C with blue ice |
| Concentration | Lot specific, generally ≥ 5.0 mg/ml |
| Shelf Life | 12 months from the date of receipt if stored as recommended |
| Formulation | PBS Buffer, PH 7.2, with no carrier protein, or preservatives. |
| Sterility | 0.2 μM filtered |
| Endotoxin | ≤ 1.0 EU/mg, by the LAL method |
| Purity | 99% |
Sade-Feldman, Moshe et al. "Defining T Cell States Associated with Response to Checkpoint Immunotherapy in Melanoma",Cell 175.4 (2018): 998-1013.e20.
Benci, Joseph L. et al. "Opposing Functions of Interferon Coordinate Adaptive and Innate Immune Responses to Cancer Immune Checkpoint Blockade",Cell 178.4 (2019): 933-948.e14.
Cappellesso, Federica et al. "Targeting the Bicarbonate Transporter SLC4A4 Overcomes Immunosuppression and Immunotherapy Resistance in Pancreatic Cancer",Nature cancer 3.12 (2022): 1464-1483.
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