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CD8b is also known as Lyt-3.2 or Ly-3.2. It is a member of the Ig superfamily expressed as a heterodimer with the CD8A chain on a subset of MHC class I-restricted T cells and most thymocytes.
| Catalog | IV0117 |
| Product Name | InVivoPro Anti-Mouse CD8β (Lyt 3.2) in vivo antibody, Clone 53-5.8 |
| Size | 1mg/5mg/25mg/50mg/100mg |
| Isotype | Rat IgG1 κ |
| Clone | 53-5.8 |
| Target | CD8β |
| Other Names | Lyt-3.2, Ly-3.2 |
| Isotype Control Catalog Code | Rat IgG1 Isotype Control |
| Dilution Buffer | PBS, pH 7.2, contains no stabilizers or preservatives |
| Reactivity | Mouse |
| Host Species | Rat |
| How much antibody to use in vivo | 200 μg per mouse; or 10 mg/kg. This range is based off the most recent publication data using the 53-5.8 clone in vivo. Each investigator should determine their own optimal working dilution for specific applications. |
| Background | CD8b is also known as Lyt-3.2 or Ly-3.2. It is a member of the Ig superfamily expressed as a heterodimer with the CD8A chain on a subset of MHC class I-restricted T cells and most thymocytes. |
| Applications | In vivo CD8+ T cell depletion,Flow cytometry, Western blot |
| Purification | protein A or G |
| Storage | This antibody is stable for at least 2 months when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at -20°C or -80°C. Avoid repeated freeze thaw cycles. |
| Shipping | 2-8°C with blue ice |
| Concentration | Lot specific, generally ≥ 5.0 mg/ml |
| Shelf Life | 12 months from the date of receipt if stored as recommended |
| Formulation | PBS Buffer, PH 7.2, with no carrier protein, or preservatives. |
| Sterility | 0.2 μM filtered |
| Endotoxin | ≤ 1.0 EU/mg, by the LAL method |
| Purity | 99% |
Brunck, Marion, et al. "Cervical Cancer-Associated Human Papillomavirus 16 E7 Oncoprotein Inhibits Induction of Anti-Cancer Immunity by a CD4+ T Cell Dependent Mechanism." Nature Precedings (2009): 1-1.
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