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CD8, a.k.a Lyt-2, Ly-2, or T8, consists of disulfide-linked A and β chains that form the A(CD8a)/β(CD8b) heterodimer and A/A homodimer. CD8a is a 34 kD protein that belongs to the immunoglobulin family. The CD8 A/β heterodimer is expressed on the surface of most thymocytes and a subset of mature TCR A/β T cells.
| Catalog | IV0119 |
| Product Name | InVivoPro Anti-Mouse CD8α in vivo antibody, Clone 53-6.7 |
| Size | 1mg/5mg/25mg/50mg/100mg |
| Isotype | Rat IgG2a κ |
| Clone | 53-6.7 |
| Target | CD8α |
| Other Names | CD8 alpha chain，Ly 2.2，Ly-35 |
| Isotype Control Catalog Code | IV0105 or Rat IgG2a Isotype Control |
| Dilution Buffer | PBS, pH 7.2, contains no stabilizers or preservatives |
| Reactivity | Mouse |
| Host Species | Rat |
| How much antibody to use in vivo | 200 μg per mouse; or 10 mg/kg. This range is based off the most recent publication data using the 53-6.7 clone in vivo. Each investigator should determine their own optimal working dilution for specific applications. |
| Background | CD8, a.k.a Lyt-2, Ly-2, or T8, consists of disulfide-linked A and β chains that form the A(CD8a)/β(CD8b) heterodimer and A/A homodimer. CD8a is a 34 kD protein that belongs to the immunoglobulin family. The CD8 A/β heterodimer is expressed on the surface of most thymocytes and a subset of mature TCR A/β T cells. |
| Applications | In vivo CD8+ T cell depletion, Flow cytometry, Western blot |
| Purification | protein A or G |
| Storage | This antibody is stable for at least 2 months when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at -20°C or -80°C. Avoid repeated freeze thaw cycles. |
| Shipping | 2-8°C with blue ice |
| Concentration | Lot specific, generally ≥ 5.0 mg/ml |
| Shelf Life | 12 months from the date of receipt if stored as recommended |
| Formulation | PBS Buffer, PH 7.2, with no carrier protein, or preservatives. |
| Sterility | 0.2 μM filtered |
| Endotoxin | ≤ 1.0 EU/mg, by the LAL method |
| Purity | 99% |
Cao, Thai M et al. "A chromosome 16 quantitative trait locus regulates allogeneic bone marrow engraftment in nonmyeloablated mice". Blood vol. 114,1 (2009): 202-10.
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